The Effect of Pyridostigmine as an Antidote for Botulinum Type A: An Experimental Study on Rabbits

Background: Botulinum toxin (BoNT) or Botox injections are popular non-surgical and non-invasive option to optimize change an individual’s facial appearance achieve rejuvenation. Pyridostigmine is acetylcholinesterase inhibitor important in clinical practice. This study evaluated the toxic effect of BoNT demonstrate efficacy anticholinesterase pyridostigmine. Methods: We conducted experimental interventional evaluate antidote pyridostigmine against BoNT. Forty rabbits (skeletally mature males, body weight 1000–2000 g) were included study. The injected with Clostridium botulinum type A neurotoxin complex (BoNT Botox), which was reconstituted 0.9% sterile sodium chloride a concentration 100 U/2.5 mL. Animals divided randomly into four groups (10 each) as follows: Group 1 (control group), group 2 (pyridostigmine-treated 3 only 4 + treated group). Results: In control group, there statistical difference between rabbits’ (P=0.03). left muscle significantly heavier than right (P=0.025). In had high during weeks (P=0.002). injection statistically significant (P=0.033), more first week (1.55±0.2 kg) then subsequently decreased fourth (1.345±0.13 kg). mean quadriceps 6.573±1.3 g, lower (8.09±1.2 P=0.0001). Conclusions: To best our knowledge, this Iraq investigate injections. saline caused increased comparison that received can act strong toxicity.